Abstract: Lime was used as the depressant of pyrite in a copper-zinc-sulfur polymetallic ore concentrator in Sichuan，in which exists some problems such as complicated preparation process of lime liquid， high single consumption and larger difficult in safety and environmental protection work. In order to practice the concept of green mine construction and sustainable development，novel inhibitor S-601 was selected to carry out substitution test. The results showed that S-601 appeared a high-efficiency inhibition effect on pyrite in the test. Under the condition of dosage of 800 g/t，the grade of copper concentrate is increased by 0.81 percentage points ，and the recovery rate is increased by 0.43 percentage points，zinc concentrate grade increased by 3.11 percentage points，recovery rate increased by 0.23 percentage points，sulfur concentrate grade increased by 0.61 percentage points，recovery rate increased by 3.63 percentage points. S-601 can be directly prepared into 2.5% solution for adding，the preparation and adding method are simple，S-601 is used as an inhibitor to replace lime，not only significantly simplified the process，saving human and material resources，and is safety and environmental protection，tons of raw ore production costs also decreased by 2.14 yuan. Therefore，the use of S-601 achieved cost reduction and efficiency increase significantly.

Key words: novel inhibitor S-601, replacing lime, efficient, environmental protection, economic benefits